Cornous Biology | Volume 4 Issue 3 | Pages: 1-7 | Doi : 10.37446/corbio/ra/4.3.2026.1-7
Review Article
OPEN ACCESS | Published on : 31-Aug-2026

Bovine ephemeral fever virus in cattle: clinical manifestations and diagnostic approaches


  • Zaid Nadhim Mohammed
  • Ministry of Education, Babylon Education Directorate, Babylon, Iraq.

  • Mahdi Hamzah Khashan
  • Intermediate Evening School, Educational Directorate of Babylon, Al-Qasim City, Babylon, Iraq.

  • Ahmed Hamzah Mosa
  • Department of Internal and Preventive Veterinary Medicine, College of Veterinary Medicine, AL-Qasim Green University, Babylon 51013, Iraq.

Abstract

Bovine ephemeral fever (BEF) also known as three-day Sickness, is acute viral disease that primarily infects cattle and leads to severe economic losses in the livestock industry in Iraq, as well as neighbouring countries. The manifestation of the disease can lead to sudden onset of fever, lameness, reduced milk production, and general lethargy. This disease is thought to spread via arthropod vectors (principally mosquitoes and biting flies); thus, high summer temperatures and high humidity enable the disease to spread quickly from animal to animal during periods of high vector activity. Periodic outbreaks of BEF occur in Iraq, Syria, Iran, Turkey and Saudi Arabia and, therefore; epidemic preparedness is essential in this area. The diagnosis of BEF is generally determined by clinical signs, geographical location, and case history; however, laboratory tests, such as RT-PCR and ELISA, can accurately confirm infection in animals suspected of being infected with BEF. Some of the control measures that can help manage BEF are vaccination, veterinary treatment of affected animals and vector control. While BEF is associated with low mortality, the economic implications resulting from losses in productivity and high veterinary expenses are severe. BEF control must therefore be a regional effort by all stakeholders willing to share their resources for the various ways of BEF surveillance, control and vaccination as well as to contain the spread of BEF and secure food supplies for livestock products.

Keywords

Iraq, virus, BEF, PCR, disease, BEF

References

  • Abdel-Hamied, E., Elgendy, I. A., Abdel-Samad, A. G., Abd-Elrahman, A. M., & Saeed, H. E. (2026). Integrated evaluation of immune response, inflammatory biomarkers, clinical features and hemato-biochemical changes in bovine ephemeral fever-affected cattle. Irish Veterinary Journal.‏ https://doi.org/10.1186/s13620-026-00332-8

    Aly, N. E., Atwa, M. H., Abbas, A. M., Abulmagd, D. M., Salem, Z. T., & Sayed, T. A. (2023). Immunogenicity of a freeze-dried combined vaccine against Rift Valley fever and bovine ephemeral fever in cattle. Open Veterinary Journal, 13(7), 826.‏ 10.5455/OVJ. 2023. v13.i7.3

    Benevenia, R., Lelli, D., Moreno, A., Lavazza, A., Kapri-Pardes, E., Klement, E., ... & Pezzoni, G. (2024). Development of two competitive ELISAs based on monoclonal antibodies for the serological detection of bovine ephemeral fever virus. Journal of Virological Methods329, 115009.‏ DOI: 10.1016/j.jviromet.2024.115009

    Carew, J., Simpson, R., Pillay, T. D., Desai, A., Toalster, R., Parkes, P., & Abbara, A. (2025). Vector-borne diseases and the Syrian conflict: A systematic review of literature from Syria and neighbouring, refugee-hosting countries. PLoS Neglected Tropical Diseases19(11), e0013721.‏ https://doi.org/10.1371/journal.pntd.0013721

    Faiz, S., Khatoon, A., Rizvi, F., & Saqib, M. (2026). First molecular evidence of bovine ephemeral fever virus in Sindh, Pakistan: phylogenetic insights and risk factor correlation.‏ Veterinary Research Forum17(7), 461-470. doi: 10.30466/vrf.2025.2066094.4842

    FAO, O. (2021). GF-TADs Strategy for 2021–2025. https://doi.org/10.20506/GFTADS.3269

    Ghanem, A. M., Alnashwan, O. S., Alqunaibet, M. H., Alduwais, A. A. M., Almodarra, S. F., & Alaagib, S. B. (2026). Measuring the impact of livestock development on local red meat production and food security in Saudi Arabia. Sustainability18(4), 1883.‏ https://doi.org/10.3390/su18041883

    Gharban, H. A., Al-Ghuraibawi, H. N., Al-Rubaye, Z. A., Jahlol, H. A., Al-Zergany, A. A., & Al-Abedi, G. J. (2023). Most clinically detected viral diseases in field animals of Wasit Province, Iraq. Annals of the Romanian Society for Cell Biology27(01), 154-168.

    Kaya, Y., & Bulut, O. (2024). The Past, Present and Future of Three-Day Sickness with Epidemiological Data. Animal Health Production and Hygiene13(2), 52-62.‏ https://doi.org/10.53913/aduveterinary.1437009

    Lavon, Y., Ezra, E., Friedgut, O., & Behar, A. (2023). Economic aspects of bovine ephemeral fever (BEF) outbreaks in dairy cattle herds. Veterinary sciences10(11), 645.https://doi.org/10.3390/vetsci10110645

    Macrae, A. (2026). Housing in dairy cattle farms. In Encyclopedia of Livestock Medicine for Large Animal and Poultry Production (pp. 596-605). Cham: Springer Nature Switzerland. https://doi.org/10.1007/978-3-031-61549-8_46

    Naderian, A., Bakhshesh, M., & Ebrahimi-Jam, M. H. (2025, September). Molecular epizootiology of bovine ephemeral fever virus in Iran during 2015 to 2022. In Veterinary Research Forum (Vol. 16, No. 9, p. 529).
    DOI: 10.30466/vrf.2025.2040560.4446

    Özyörük, F., Özgünlük, İ., Turgut, S. İ., Şengül, Y., Yığman, A. A., & Gülyaz, V. (2025). Environmental and Breed‐Related Determinants of Bovine Ephemeral Fever Outbreaks in Southeastern Türkiye. Veterinary medicine and science, 11(2), e70257.‏ https://doi.org/10.1002/vms3.70257

    Paksoy, N., Balıkçı, C., Merhan, O., Dinçer, E., Şahan, A., & Bozukluhan, K. (2024). Evaluation of acute phase response in cattle with naturally infected bovine ephemeral fever virus. Acta Veterinaria Brno93(2), 129-134. https://doi.org/10.2754/avb202493020129

    Payami, M., Aghighi, M. E., Safari, N., Akbari, T., Sayahi, A., Shourehdeli, P. I., ... & Shams, F. (2026). Global prevalence and epidemiological dynamics of bovine ephemeral fever: a misclassification-adjusted bayesian meta-analysis. BMC Veterinary Research.‏ DOI: 10.1186/s12917-026-05529-1

    Rezatofighi, S. E., Mirzadeh, K., & Mahmoodi, F. (2022). Molecular characterization and phylogenetic analysis of bovine ephemeral fever viruses in Khuzestan province of Iran in 2018 and 2020. BMC veterinary research18(1), 19. DOI: 10.1186/s12917-021-03119-x

    Solanki, S., Manu, M., Mishra, A., Bora, M., Mahajan, V., & Gupta, K. (2025). Development of a TaqMan based real-time PCR assay for the detection of bovine ephemeral fever virus. Molecular Biology Reports52(1), 1044.
    DOI: 10.1007/s11033-025-11157-z

    Tokgoz, B. S., Tokgoz, E. A., Sözmen, M. A., Avci, O., & Ütük, A. E. (2023). Prevalence, isolation and molecular characterization of Bovine Ephemeral Fever Virus in south and southeast regions of Turkey in the outbreak of 2020. Journal of the Hellenic Veterinary Medical Society74(4), 6543-6552. https://doi.org/10.12681/jhvms.31543

    Toulba, M., Dawoud, M., Mabrouk, I., Elsify, A., Salama, A., Mousa, W., ... & Nayel, M. (2026). A Meta-Analysis of Efficacy and Immunogenicity of Bovine Ephemeral Fever Vaccines Worldwide. Journal of Current Veterinary Research8(1), 80-99. DOI: 10.21608/jcvr.2026.502188

    WOAH. (2023). Global strategy for diagnostics in transboundary animal diseases. World Organisation for Animal Health.

    Yilmaz, A., Umar, S., Turan, N., Kayar, A., Richt, J., & Yilmaz, H. (2022). Current scenario of viral diseases and vaccination strategies of cattle in Turkey. Journal of Infection in Developing Countries16(8). DOI: 10.3855/jidc.14767