The quest for plants with medicinal properties continues to receive attention as scientist survey plants, particularly with ethnobotanical, significance for complete range of biological activities ranges from antibiotic to anti-tumor. The anti-microbial compounds present in medicinal plants are degradable and selective in their toxicity and so they are considered as valuable ingredients for controlling plant diseases caused by virus, bacteria and fungi. The antifungal compounds of two medicinal plants viz., Lantana camara and Bougainvillea glabra was extracted by using ethyl acetate. Active spots identified by thin layer chromatography assay and were further purified using preparative HPLC. The purified compounds were subjected to chemical analysis and found to contain phenolic group of compounds. HPLC purified Lantana camara ethyl acetate extract was found to be more active against Pythium aphanidermatum than the Bougainvillea glabra extract. The compound was further analysis and confirmed by GCMS. The partially purified phenolic compounds can be used against plant pathogens. However further study in the purified compounds must be under taken to validate the results.
plant species, pathogens, chromatogram, chemical compounds
Akhtar, M. A., M. H. Rahber Bhatti and M. Aslam. 1997. Antimicrobial activity of plant diffusates against Xanthomonas campestris pv citri. Int. J. Pest. Mgt., 49:
Anil, D., S.Srivastava and M. Shaija. 2006. Antimicrobial activity of Latana camara plant extract. Central drug research insistute.
Babu, S., K. Seetharaman, R. Nandakumar and J. Johnson. 2001. Fungi-toxic properties of some plant extract against Alternaria solani, the tomato leaf pathogen.
J. Ecotoxicol. Envt. Monitoring 10: 157-159.
Balasaraswathi, R.1995. Isolation and characterization of anti-viral principals from plant extract with special reference to Bougainvillera spectabilis wild against tomato spotted wilt virus. Ph.D Thesis. Bharathiar University Coimbatore, India. Pp: 172.
Barre J., T. Bowden, J.C Coll and G.C. Janairo. 1997. A bioactive triterpene from Lantana camara. Phytochemistry 45 (2): 321-324.
Bindu, T.K., P. M. Shati, P.P. Rajan and V. R. Sharma. 1998. Antifungal activity of Uvaria narum extracts. J. Allelopathy 35: 67-74
Bonjar, 2004.Screening of some medicinal plants for their antimicrobial activities. J.Ethnopharmocolog 62:183-193.
Claeys,M., L.Pieters, J.Corthout, D.A.Vanden berghe and A.J.Vlietinck .1988. A new antimicrobially active flavonoid from Lantanna trifolia. Journal of Natural Products 51 (5): 966-968.
Hahn, D.D., J.M. Faubion and L.W. Rooney.1993. Sorghum phenolic acids their high performance liquid chromatographic separation and their relation to fungal resistance. Cereal Chem. 60(4):255.
Kumbhar,.P.,M., Kshama, B.Chawan, R.S.Nikubhbendre and N.M. Dewing. 2001. Antifungal and repellent potency of some medicinal plant extract. Pestology 25: 44-46.
Pansey,V.G and S.V.Sukhatme. 1965. Statistical method for agricultural workers. Indian Council of Agriculture Research, New delhi., p.359
Phongpaichit, S., N. Pujenjob, V. Ruka Chaisirikul and M. Engsakul. 2004. Antifungal activity from leaf extracts of Cassia alata L., Cassia fistula L. and Cassia tora L. J. Sci. Technol 26(5): 741-748.
Rao M.R., I.B. Reddy and Ramana. 2006. Antimicrobial activity of some Indian medicinal plants. Indian Journal Microbiology 46(3): 259-262.
Sabitha, A., and O. Suranarayana. 2004. Antifungal potential of flower head extract of Spilanthes acmella linn. African Journal of Biomedical Research 9: 67-68.