Background: The interest in spirulina lies due to its high level of protein and mineral content besides its excellent functionality and health promoting characteristics.
Methods: Spirulina powder was added to milk at different levels 0.3 and 0.5% respectively. Analysis of variance (ANOVA) with a least significant difference (LSD) test was applied for multiple sample comparisons to test for any significant differences (p≤0.05) in the mean values of all the groups.
Results: The average chemical composition of spirulina powder showed protein 68%, fat 6%, ash 9%, moisture 3%, carbohydrate (by difference) 14%, Iron 980 ppm and calcium 685 ppm. Gamma Linolenic acid (GLA) was found as a major fatty acid i.e.30mg/gm in spirulina powder.
Conclusion: An increase in protein content 0.76 %, essential fatty acid 8.02%, iron 5.16 ppm and calcium 137.37ppm, and gamma Linolenic acid at both the levels of spirulina powder addition in milk was observed when compared with control during the storage at refrigeration temperature over a period of 6 days. Separation of milk protein containing spirulina powder was carried out using SDS-PAGE. It showed that milk protein bands interact with spirulina proteins near 19 kDa.
flavoured milk, spirulina powder, storage study, sensory characteristics
Ackman, R. G., Ratnayake, W. M. N., & Olsson, B. (1988). The “basic” fatty acid composition of Atlantic fish oils: potential similarities useful for enrichment of polyunsaturated fatty acids by urea complexation. Journal of the American Oil Chemists' Society, 65(1), 136-138.
Amerine, M. A., Roessler, E. B., & Ough, C. S. (1965). Acids and the acid taste. I. The effect of pH and titratable acidity. American Journal of Enology and viticulture, 16(1), 29-37.
Benedetti, S., Marelli, M., & Candela, M. (2020). Potential health benefits of spirulina for cardiovascular and metabolic diseases. Marine Drugs, 18(12), 618.
Benedetti, S., Canali, R., & Rossi, M. (2023). Potential of phycocyanin for brain health: A narrative review. Nutrients, 15(3), 522.
Calder, M. M. (2015). Marine omega-3 fatty acids and neural development in children. The American Journal of Clinical Nutrition, 103(6), 1594S-1602S.
Calder, P. C. (2015). Marine omega-3 fatty acids and inflammatory processes: Effects on rheumatoid arthritis. Current Opinion in Rheumatology, 27(1), 3–10.
Chamorro, G., Salazar, M., Favila, L., & Bourges, H. (1996). Pharmacology and toxicology of Spirulina alga. Revista De Investigacion Clinica; Organo Del Hospital De Enfermedades De La Nutricion, 48(5), 389-399.
Chen, J., Li, Y., Wang, C., Li, S., & Zhang, J. (2022). Nutritional composition and health benefits of Spirulina. Journal of Food Science and Technology, 59(12), 5833–5842.
Clement, J. S., Oliveira, M. M., Mendes, B. S., & Melo, F. V. T. R. (2020). Potential benefits of spirulina in cognitive decline and dementia.
Clement, M., Mateo, J., & Ruiz, L. (2022). Phycocyanin: A promising marine natural product with potential therapeutic applications in neurodegenerative diseases. Frontiers in Pharmacology, 13, Article 824222.
Dewit, J.N., & Swinkel, G.A.M.(1980). Structure and functional behaviour of whey proteins. Neth. Milk Dairy J.,35, 47-64.
Dewey, K. G. (2001). The future of breastfeeding research. The Journal of Nutrition, 131(2), 429S-433S.
Freindenreich, P., Apell, G. S., & Glazer, A. N. (1978). Structural studies on phycobiliproteins (II). J Biol Chem., 253, 212-9.
Harrigan, W.F, Mac-Cance, M.E. (1976) Laboratory methods in food and dairy microbiology. Academic Press, London.
Harrigan, W.F. (1998) Laboratory methods in food microbiology (3 rd Edn). Academic Press,London.
Hau R, (1995), “Vitamin B12 in der Mikroalge Spirulina platensis” FIT fürs LEBEN 1, 29.
Huang, S., Xiong, Y., Jiang, M., Ren, J., & Li, H. (2021). Consumer preferences for fortified milk beverages in China: A case study in Shanghai. Journal of Dairy Science, 104(11), 11245–11254.
Innis, S. M. (2007). Dietary omega-3 fatty acids and the developing brain. The American Journal of Clinical Nutrition, 85(3), 608S-611S.
Iverson, S. J., Lang, S. L., & Cooper, M. H. (2001). Comparison of the Bligh and Dyer and Folch methods for total lipid determination in a broad range of marine tissue. Lipids, 36(11), 1283-1287.
Jimenez-Corona, M. E., García-Valdés, P. A., Moustafa, M. E., & Gamal-Eldeen, E. E. (2023). Applications of Spirulina (Arthrospira): A review of its potential in the food and pharmaceutical industry. Journal of Food Quality, 46(2), Article e26924.
Khan, M. A., Zhao, Y., Li, B., Wu, S., & Liu, Z. (2022). A comprehensive review on the health benefits and applications of Spirulina. Trends in Food Science & Technology, 122, 130-142.
Kong, W., Liu, G., Lv, Y., Gong, C., Li, J., & Xia, C. (2022). Antioxidant and anti-inflammatory activities of Spirulina platensis extracts. International Journal of Molecular Sciences, 23(13), 7152.
Leaf, A., & Weber, P. C. (1988). Cardiovascular effects of n-3 fatty acids. New England Journal of Medicine, 318(9), 549-557.
Liu, Z., Zhu, Q., Huang, D., & Zhang, A. (2016). Potential health benefits of phycocyanin. Current Pharmaceutical Design, 22(14), 2081-2088.
Li, Y., Liu, X., Chen, J., Chen, X., & Zhang, J. (2021). The effects of Spirulina platensis supplementation on exercise performance: A meta-analysis of randomized controlled trials. Nutrients, 13(1), 189.
Liu, L., Xu, L., Wang, Y., & Liu, Z. (2021). γ-Linolenic acid: A nutritional nutraceutical with multifaceted therapeutic benefits. Frontiers in Pharmacology, 12, Article 681323.
Malik, P. 2013.Quality characteristics of ice cream enriched with spirulina powder.International Journal of food and Nutritional Science.
MacColl, R. (1983). Stability of allophycocyanin's quaternary structure. Archives of Biochemistry and Biophysics, 223(1), 24-32.
Metin, O., Mazumder, V., Ozkar, S., & Sun, S. (2010). Monodisperse nickel nanoparticles and their catalysis in hydrolytic dehydrogenation of ammonia borane. Journal of the American Chemical Society, 132(5), 1468-1469.
Patel, A., Mishra, S., & Ghosh, P. K. (2006). Antioxidant potential of C-phycocyanin isolated from cyanobacterial species Lyngbya, Phormidium and Spirulina spp.
Ramasamy, D., Shibu, A.V., & Gopi, H. (1999). Dairy technologist handbook, Lucknow: International book distribution company, pp 72-73.
Ranganna,S.2005.Ch.5- Vitamins and Ch. 28 – Fruit juices, concentrates and beverages. In:Handbook of analysis and quality control for fruit and vegetable products,2 nd ed. Published by Tata McGraw-Hill Publishing Company Limited, New Delhi, India. pp 105-106,868-882,891.
Rao, M. B., & Reddy, T. S. (2007). Brain composition and function: a central role for phospholipids.
Roy,N.K. & Sen, D.C.1994.Chemical Analysis of Fluid Milk. In: Textbook of Practical Dairy Chemistry,Vol.-1,2 nd ed., Kalyani Publishers, New Delhi, India. pp 22-25.
Sanchez, M., Bernal-Castillo, J., Rozo, C., & Rodríguez, I. (2003). Spirulina (Arthrospira): an edible microorganism: a review. Universitas Scientiarum, 8(1), 7-24.
Shahidi, F. (2009). Omega-3 (n-3) fatty acids in health and disease. AOCS Press.
Silva, L. L. S., Augusto, R. A., Tietzmann, D. C., Sequeira, L. A. S., Hadler, M. C. C. M., Muniz, P. T., ... & ENFAC Working Group. (2017). The impact of home fortification with multiple micronutrient powder on vitamin A status in young children: A multicenter pragmatic controlled trial in Brazil. Maternal & Child Nutrition, 13(4), e12403.
Simopoulos, A. P. (2001). The importance of the omega-6/omega-3 fatty acid ratio in breastfeeding and infant feeding. The Journal of the American College of Nutrition, 20(4), 532S-542S.
Simopoulos, A. P. (2004). Omega-3 fatty acids in inflammation and autoimmune diseases. The Journal of the American College of Nutrition, 23(5), 490S-495S.
Snedecor, J. Roy & Cochran, W. G. (1994). Journal of Educational and Behavioral Statistics 19 (3), 304-307.
Tokusoglu, O., & Unal, M. K. (2003). Fat replacers in meat products. Pakistan Journal of Nutrition, 2(3), 196-203.
Vieira Costa, J. A., Batista, A. P., Mendonça, R. A., & Coelho, M. A. (2015). Gamma-linolenic acid: physiological effects and therapeutic potential. Química Nova, 38(8), 891-900.
