This study aimed to assess the phytochemical profile and quantify the total phenolic and flavonoid content of Brussels sprouts under two environmental conditions. The phytochemical screening of methanol, aqueous and hydro alcohol extracts of different Brussels sprouts genotypes under open and protected environments was conducted as per standard methods. The total phenolic content was measured by the Folin–Ciocalteu assay and total flavonoid content was measured using the Aluminum chloride method. Phytochemical screening was performed to confirm the occurrence of essential bioactive compounds and results revealed significant variations in phytochemical compositions, phenolic and flavonoid content between the two environments, indicating the influence of environmental factors on secondary metabolites accumulation. Genotype Hild’s Ideal under open environment had the highest phenolic content (26.32 mg GAE/g) with methanol extract, while genotype Urja had the lowest value (6.87 mg GAE/g) with aqueous extracts. The highest total flavonoid content was recorded in genotype Hild’s Ideal (13.92 mg QE/g) using methanol extracts under a protected environment; whereas, the lowest flavonoid content 4.29 mg QE/g, was observed in genotype Urja with aqueous extracts under an open environment. Methanol was identified as the most effective solvent for the extraction of the phenolic and flavonoid compounds. The presence of bioactive compounds suggests potential health benefits, supporting the use of Brussels sprouts as an important medicinal and functional vegetable.
Brussels sprouts, phytochemical screening, environment, phenolic, flavonoid content
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