Journal of Innovative Agriculture, Volume 12, Issue 3 : 10-17 . Doi : 10.37446/jinagri/rsa/12.3.2025.10-17

Research Article

OPEN ACCESS | Published on : 30-Sep-2025

Analysis of chlorophyll mutations in EMS-induced mutant population of rice (Oryza sativa L.)

Balu Indragandhi Hariharan
• Department of Genetics and Plant Breeding, SRM College of Agricultural Sciences, SRM Institute of Science and Technology, Chengalpattu-603 201, Tamil Nadu, India.

Jegadeeswaran Mokkaraj
• Department of Genetics and Plant Breeding, SRM College of Agricultural Sciences, SRM Institute of Science and Technology, Chengalpattu-603 201, Tamil Nadu, India.

P. Chandrasekaran
• Department of Basic Sciences, SRM College of Agricultural Sciences, SRM Institute of Science and Technology, Chengalpattu-603 201, Tamil Nadu, India.

Arulbalachandran Dhanarajan
• Division of Crop Stress Physiology and Molecular Biology, Department of Botany, School of Life Sciences, Periyar University, Salem, Tamil Nadu, 636011, India.

Thirugnana Kumar S
• Department of Genetics and Plant Breeding, SRM College of Agricultural Sciences, SRM Institute of Science and Technology, Chengalpattu-603 201, Tamil Nadu, India.


• Selvakumar Gurunathan
• Department of Genetics and Plant Breeding, SRM College of Agricultural Sciences, SRM Institute of Science and Technology, Chengalpattu-603 201, Tamil Nadu, India.

Abstract

Background: The purpose of this study was to determine the lethal dose for chemical mutagen ethyl-methane sulfonate (EMS) in the high-yielding rice variety ADT43. Secondly, to analyse the frequency of chlorophyll mutations in the M₂ generation.

Methods:  In this study ten batches of rice seeds treated with different dosages of ethyl methane sulphonate (EMS) viz., 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, and 1.0% and kill curve analysis was carried out to determine lethal doses. To create M₁ population, the EMS doses of 0.4%, 0.5%, and with a higher dose 0.6% were used. Then, 700 (0.4% EMS), 700 (0.5% EMS), and 700 (0.6% EMS) M₂ families, were subsequently raised to record the chlorophyll mutations.

Results: The LD50 value resided at 0.4% to 0.5% EMS. The lethality of the rice increased linearly with a gradual increase in the EMS dosage. Frequency of M₂ families with chlorophyll mutants recorded in the EMS doses 0.4%, 0.5%, and 0.6% were 4.00%, 4.57%, and 6.14%, respectively.

Conclusion: The presence of chlorophyll mutants in the M₂ generation was confirmed the occurrence of mutation in the mutant population during the mutagenesis. Hence, the reliability of the mutant population is verified to utilise this mutant population to screen for the trait of interest further.

Keywords

chlorophyll mutant, EMS, mutation breeding, rice

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