Background: The purpose of this study was to determine the lethal dose given by the chemical mutagen ethyl-methane sulfonate (EMS) in the high-yielding rice variety ADT43. The frequency of chlorophyll mutations has been analysed in the M2 generation.
Methods: A pilot study that consisted of treating ten batches of rice seeds using different dosages of ethyl methane sulphonate (EMS) and conducting kill curve analysis was used to determine lethal doses. The M1 population was then divided into three batches, each with EMS dosages of 0.4%, 0.5%, and 0.6%. Three batches of the M2 population, involving the 700, 700, and 700 M2 families, were subsequently exposed to chlorophyll mutation in the subsequent cropping and studied.
Result: The range of the LD50 value is 0.4% to 0.6% EMS. The lethality of the rice increased linearly with a gradual increase in the EMS dosage. At EMS dosages of 0.4%, 0.5%, and 0.6%, the rates of the chlorophyll mutation were 4, 4.57, and 6.14, respectively.
Conclusion: Since the presence of chlorophyll mutants in the M2 generation is confirmed, the reliability of the mutant population is verified to utilise this mutant population to screen for the trait of interest.
chlorophyll mutant, EMS, mutation breeding, rice
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