Journal of Innovative Agriculture, Volume 11, Issue 4 : 29-33 . Doi : 10.37446/jinagri/rsa/11.4.2024.29-33

Research Article

OPEN ACCESS | Published on : 31-Dec-2024

Haplotype analysis of OsMATL, a pollen specific phospholipase gene responsible for haploid induction

• Raveendran Muthurajan
• Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore - 641003, India.

Veera Ranjani Rajagopalan
• Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore - 641003, India.

Sakthi Ambothi Rathnasamy
• Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore - 641003, India.

Sudha Manickam
• Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore - 641003, India.

Abstract

Background: Double haploid breeding is a modern plant breeding approach that enables the rapid development of fully homozygous lines within a single generation. In rice, the creation of efficient haploid inducer lines requires the identification of novel allelic variants of the OsMATL gene across diverse germplasm. To achieve this, OsMATL sequences from rice accessions in the 3K panel were analyzed to detect unique SNPs that could potentially contribute to enhance haploid induction efficiency and support hybrid rice breeding programs.

Methods: Sequence retrieval from SNP seek database (https://snp-seek.irri.org), allelic diversity and haplotype analysis.

Results: OsMATL has 30 variations according to allelic diversity analysis. Three haplotypes (H1, H2 and H3) were identified based on three non-synonymous SNPs for MATL gene.

Conclusion: Established haplotype analysis for the MATL gene for the 783 lines belongs to 3K Rice genome panel.

Keywords

rice, OsMATL gene, allelic variation, Haplotype analysis, Haploid induction, hybrid rice breeding

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